Compute per-compound operating concentrations against your downstream assay window, minimizing aggregate-driven re-runs.
Summary
Surface Plasmon Resonance (SPR) campaigns depend on clean hit lists. Aggregators that survive into the SPR queue generate false positives, distort kinetic constants, and damage microfluidics, driving regular sensor-chip rework and full system cleaning cycles. We demonstrate a plate-based pre-SPR triage workflow on ORYL F1 that profiles entire hit lists at SPR-relevant timepoints in ~15 minutes per 384-well plate, using ~2 μL per datapoint. A 142-compound hit list, including SPR-flagged problematic compounds, was measured at two timepoints (1 h and 24 h) with eight concentrations and two replicates.
The workflow returns per-compound diagnostics: a safe operating concentration, an avoid-above threshold, peak aggregation amplitude, a timepoint-shift behavior class, and quality flags — all with bootstrap confidence intervals. F1 risk calls concord with the SPR assay’s internal severity assessments across 10 of 11 scored compounds. The per-compound output drops directly into an SPR queue as an operating-concentration input. A ROI estimator on a 1,000-compound campaign places savings on the order of ~20 kCHF per campaign in chip consumables, rework, and microfluidics maintenance.