How much does running SPR without solubility triage cost you per year? Eleven inputs, three strategies (pre-filter, SPR + resume, SPR + restart), your numbers.
A single catastrophic chip failure can invalidate hours of binding kinetics work and delay campaign decisions. Solubility and aggregation triage before SPR removes the source of these failures entirely.
Particulates and aggregated material damage sensor chip surfaces and clog SPR fluidic systems. Pre-filtering problem compounds significantly reduces catastrophic failures and unplanned service events.
Aggregators and insoluble compounds generate unreliable binding data, yet consume the same instrument time and reagent cost as clean compounds. Screen them out before they reach the SPR.
Adjust the sliders to reflect your throughput and costs.
Solubility assay sensitivity is 100%. If your assay catches only X% of problem compounds, discount the savings by that fraction.
Microfluidics damage is partly driven by problem compounds. The model assumes pre-filtering eliminates ~50% of fluidics-damage risk. The residual reflects wear, contamination, and operator error.
Catastrophic failures waste an average of half a chip. In the conservative scenario only the pre-failure batch is re-run; in the aggressive scenario the full chip batch is invalidated.
Instrument downtime, project delays, and protein supply are not priced. These factors typically strengthen the savings case further.
| Solubility assays (all compounds) | — |
| SPR runs (clean compounds only) | — |
| Planned chip consumption | — |
| Microfluidics (50% residual) | — |
| Annual total | — |
| SPR runs (all compounds) | — |
| Wasted SPR (½ chip per failure) | — |
| Chip consumption + re-prep | — |
| Microfluidics (full rate) | — |
| Annual total | — |
| SPR runs (all compounds) | — |
| Wasted SPR (full chip per failure) | — |
| Chip consumption + re-prep | — |
| Microfluidics (full rate) | — |
| Annual total | — |
Profile the hit list on the ORYL F1 upstream of SPR — aggregators are flagged before they consume sensor chips and analyst time, returning a per-compound table that drops into the SPR operating-concentration input.
Aggregators that survive into the SPR queue generate false positives, distort kinetic constants and can adsorb non-specifically onto the sensor surface and block microfluidic channels; profiling solubility and aggregation first removes them.
Pre-screening a hit list on the ORYL F1 is far cheaper per compound than SPR and avoids wasted sensor chips and analyst time; this calculator estimates the saving for your campaign size.
Our application scientists can provide a personalised ROI report based on your instrument setup and campaign throughput.