The typical output measurement is a plot of intensity vs concentration, as shown below for a known poorly soluble compound (albendazole) with n=4 replicates. As control the intensity vs concentration plot for caffeine, a soluble compound in the range of concentration tested is also shown. At concentrations below the solubility limit (<1 µM), the intensity is flat (which defines the baseline). At concentrations above the solubility limit, pre-aggregation occurs and is detected with an increase in intensity. For Albendazole, the intensity starts to increase from 1 µM and a clear jump in intensity is visible at concentration above 2.5 µM. For comparison, the intensity of caffeine stays flat, which indicates that caffeine is soluble, showing no signs of pre-aggregation as expected.