A thorough understanding of the appropriate solubility and aggregation of compounds is key for decision making, and should be obtained as early as possible in the development of a product. By assessing the solubility of your compounds, we help you make an informed decision and accelerate your research and development.
40% of drugs on the market are poorly soluble or insoluble, causing:
This enormous waste of the drug development pipeline can be avoided by a systematic assessment of solubility during R&D with high quality data.
We offer a solubility measurement service with:
| Type of measurement | Kinetic solubility |
| Solvent | Ultrapure water, PBS (alternatives available on request) |
| Final DMSO concentration | 1% |
| Number of replicates | 3 |
| Temperature | 22 °C |
| Data delivery | Estimated solubility range, curve analysis |
| Estimated delivery time | within 1–4 weeks by email |
You have specific requirements for your measurement? Please contact us and we will taylor our offer to your needs.
The typical output measurement is a plot of intensity vs concentration, as shown below for a known poorly soluble compound (albendazole) with n=4 replicates. As control the intensity vs concentration plot for caffeine, a soluble compound in the range of concentration tested is also shown. At concentrations below the solubility limit (<1 µM), the intensity is flat (which defines the baseline). At concentrations above the solubility limit, pre-aggregation occurs and is detected with an increase in intensity. For Albendazole, the intensity starts to increase from 1 µM and a clear jump in intensity is visible at concentration above 2.5 µM. For comparison, the intensity of caffeine stays flat, which indicates that caffeine is soluble, showing no signs of pre-aggregation as expected.
A typical measurement report will contain a tabulated summary of the approximate solubility values for each compound, a detailed plot and solubility analysis for each compound, the protocol used for the solubility measurement and an brief explanation of the solvent redistribution method. Additional analysis can be performed as requested.
The Solvent Redistribution method is a general and flexible method that looks at how the solvent redistributes as a function of external stimulus. The method can be performed at different pH, temperature, pressure, salt concentration, etc…
Typical negative control is caffeine (soluble compound) and typical positive controls are Amiodarone and Albendazole (poorly soluble > 1 µM).
The Solvent Redistribution method is based on high efficiency second harmonic light scattering that is sensitive to the anisotropic distribution of solvent molecules (dipolar molecules such as water, dmso, methanol, etc).