Solubility Measurement as a Service

A thorough understanding of the solubility and aggregation of compounds is key for decision making, and should be obtained as early as possible in the development of a product. At Oryl, we help you make an informed decision, accelerate your research and development and save compounds.

Minimal quantity

~2 μL of stock solution per solubility datapoint

Sensitive and accurate

1 µM (MW < 500 Da) and low error (std/mean < 5 %)

Rapid and high-throughput

1-100 ms read time per well, automated process

How does it work?

Sample Preparation

Plating of compounds

Addition of solvent

The customer plates the compounds in 384 or 96 well-plates with an acoustic robot (Echo, Labcyte) and sends the plates to Oryl; or the customer sends the compounds to Oryl and Oryl plates the compounds with an acoustic robot.


Direct screening and analysis

Well-plates are directly tested using the Solvent Redistribution method or incubated during a customer-defined incubation time (typically 2 hours) prior to testing.


Report generation and feedback

Reports are generated within 1 week of receipt of samples.

Protocol details

Type of measurementKinetic solubility (1% final DMSO concentration)
Liquid dispensingEcho platform (2.5 nL droplet)
Compound consumption~2 µL of 10 mM stock solution per solubility datapoint
Precisitionstd/mean < 5%
Sensitivity< 1 µM
Throughput384 wellplate in 13 minutes
Data deliveryEstimated solubility range, curve analysis
SolventStandard PBS buffer (alternatives possible: change of pH,
with excipients, etc.)

You have specific requirements for your measurement? Please contact us and we will adjust our offer to your needs.

Solubility measurements with the Solvent Redistribution method

Solubility measurements comparing the Solvent Redistribution method, laser-based nephelometry and HPLC-UV based methods. For full details of the benchmarking study, visit this page.

Example solubility analysis for 3 replicates of a given compound. The solubility limit is calculated as the concentration at which the threshold is reached. The threshold is defined as mean + 3 * standard deviation of the baseline, with the baseline defined as the intensity of the 2 lowest concentrations.

Frequently Asked Questions