The typical output measurement is a plot of intensity vs concentration, as shown below for a known poorly soluble compound (albendazole) with n=4 replicates. A control plot of intensity vs concentration is also shown for caffeine, a compound that is soluble in the range of concentration tested.
At concentrations below the solubility limit (<1 µM), the intensity is low and constant (which defines the baseline). At concentrations above the solubility limit, pre-aggregation occurs and is detected with an increase in intensity. For albendazole, the intensity starts to increase from 1 µM and a clear jump in intensity is visible at concentration above 2.5 µM. For comparison, the intensity of caffeine stays flat, which indicates that caffeine is soluble within this range of concentrations, showing no signs of pre-aggregation as expected.