Last week we showed what the ORYL F1 workflow looks like. This week is the data behind it. The question we had to answer: can Second Harmonic Scattering move from a specialist optical bench into a practical lab workflow without losing measurement quality?
Application Note AN-1001 reports the answer — and it’s not just that quality is preserved. The ORYL F1, the first commercially available microtiter well-plate-based SHS instrument, slightly beats the state-of-the-art goniometric reference on both R² and RMSE, while measuring more than 200 times faster and using 10 times less sample.
Why H₂O/D₂O is the right calibration system
Substituting hydrogen with deuterium produces a measurable change in SHS intensity: D₂O gives ~40% higher SHS signal than H₂O, linked to its stiffer hydrogen bond network. That response is well-characterised, reproducible, and linear across the full concentration range — which makes H₂O/D₂O mixtures an ideal calibration standard for benchmarking SHS sensitivity. AN-1001 runs 11 mixture concentrations (0–100% D₂O) on both ORYL F1 and a state-of-the-art goniometric setup operating in a laser class 4 safety laboratory.
What the comparison shows
The goniometric reference produces R² = 0.95 and RMSE = 0.0257. ORYL F1 produces R² = 0.98 and RMSE = 0.0207 — slightly better on both metrics. Average coefficient of variation across 8 replicates: 2.24%. The correct D₂O/H₂O intensity ratio of 1.40 is reproduced accurately.
The throughput comparison is stark. The goniometric setup takes more than 66 minutes to measure 11 samples, plus over 11 minutes of manual handling time between vials. One replicate of 11 samples on ORYL F1 takes 21.2 seconds — no manual handling required. The total run of 8 replicates completed in under 4 minutes. That’s more than 200 times faster, with 10 times less sample, and no laser class 4 safety laboratory required.
What this means for the rest of the data
AN-1001 is the instrument validation that underpins every application note that follows. It establishes that the same measurement quality available on a specialist optical bench is fully preserved in a 384-well plate format — and that the platform is ready for high-throughput pharmaceutical applications. It’s worth reading before you evaluate the application data.
Measurement quality preserved. Throughput transformed.