Detect precipitation and degradation in your 10 mM DMSO compound library at plate scale — with no chemistry-specific reagents and no HPLC reference standards.
Summary
Compound management teams store large libraries as nominal 10 mM DMSO stocks, but not every stock remains fully dissolved during storage and handling. When aggregates are already present in the stored stock, the dissolved fraction can fall below the intended concentration and introduce avoidable uncertainty into downstream screening workflows. This application note describes a high-throughput, low-compound, plate-based workflow on ORYL F1 for Go/No-Go QC of stored compound stocks using complementary second harmonic scattering (SHS) and linear light scattering (LLS) readouts.
Compounds were loaded directly into an ECHO low-dead-volume plate and scanned non-destructively on the ORYL F1. The two readouts were interpreted together to provide a robust and reliable QC call. When SHS and LLS both support the same conclusion, confidence in the result is increased. When one readout, e.g. SHS, is affected by sample optical properties such as a green-colored stock, the complementary readout, e.g. LLS can be used to support the classification. Visual inspection of the stock wells was used as an orthogonal validation layer.
Once plates are ready, 384 wells can be measured in approximately 15 minutes. The workflow is designed as a practical first-pass QC step for compound management teams that need decision-ready results before assay deployment or compound redistribution, helping de-risk downstream screening and reduce avoidable rework.