EASY – Easy Assessment of SolubilitY

EASY Screener

Direct measurement of aggregation for solubility
  • Simplified, cost-efficient & reliable solubility measurement
  • Maximize time savings
  • Avoid repetitive and labor-intensive work
  • Sustainable use of compounds & chemicals
  • Straightforward analysis

For faster, better and effective drug development!

As simple as nephelometry and as reliable as LC-MS

How does it work?




Simplifying Solubility Measurement

State-of-the-art (LC-MS)

ORYL process

By removing the problematic steps, Easy saves


TIME (5x)


COST (20x)





UN Sustainable Development Goals

Calibration using mixtures of heavy (D2O) and light (H2O) water

H2O Content (%)

H2O content (%)Std/Mean (%)
100 %2.3%

Direct measurement of aggregation for solubility

What is the technology behind the EASY Screener?

The EASY Screener is a light-scattering device that is different from the conventional linear scattering or the popular dynamic light scattering. The EASY Screener is based on the physics of nonlinear light scattering where an ultrafast laser that generates very high peak intensities is used to excite the sample. In particular, the device is extremely sensitive to how substances (proteins, compounds, macromolecules) aggregate at the pre-aggregation level and is a direct measurement of aggregation with molecular level information. As proof of sensitivity we calibrate the device with mixtures of D2O (heavy water) and H2O (light water). The device is able to differentiate <2% change in D2O content. With this precision, detecting aggregation of small compounds is straightforward.

Sensitive to changes in molecular structure

Differentiates ~2% change of D2O

Designed to measure solubility

Easily handles small compounds (<1 nm) and beyond

Excellent repeatability & reproducibility

Relative error (std/mean) < 5% 

EASY is designed to measure the solubility limit

Comparison with nephelometry

We tested several compounds (in duplicate) that are poorly soluble (<50 µM solublity limit) and performed a direct comparison with nephelometry with the same experimental conditions. As a metric, we use the relative error (std/mean) to determine the reproducibility of the measurements.

Summary of results

The table below shows the measured solubility limit for the compounds tested comparing the EASY Screener and nephelometry:

CompoundEASY (µM)Nephelometry (µM)
Compound 1>551
Compound 2>2590
Compound 3>1027
Compound 4>2.527

Key Highlights

  • EASY Screener detects solubility limit at lower concentration than nephelometry
  • The EASY Screener produces precise and reproducible measurements at low concentrations (std/mean < 5%)
  • Nephelometry is not suitable to obtain reproducible measurements at low concentrations (std/mean is large)
  • EASY is extremely useful for investigating dynamics at the pre-aggregation level.

EASY – Compound 1

Concentration (µM)

Y-axis is normalized to the average intensity of the first two concentrations: 0.1 and 1 µM concentration which defines the baseline. The blue/black plots are duplicate measurements, orange is reference (soluble) compound.

EASY with precise and reproducible measurements

Concentration (µM)Std/Mean (%)
252.7 (saturated)

The solubility limit is visible as a jump in the normalized intensity (normalized to the average of the first two datapoints). The two plots (black, blue) are duplicate measurements of known compound that is insoluble. As reference, the response of a known compound that is soluble (orange plot) is shown. The response of the soluble compound is flat, indicating that the there are no aggregates (i.e., exists in monomer form) in the range of concentrationd tested. The solubility limit is defined as the concentration just before the  normalized intensity is above 150% of the baseline, here, the solubility limit is detected at 5 µM. The table shows the relative error for each concentration (N=30 acquisitions). The relative error is < 5% at low concentrations showing excellent repoducibility for the EASY screener.


Concentration (µM)

Y-axis is nephelometric units subtracted with the intensity of a blank well-plate (negative values are possible). The measurements were done with N=5 replicates.

Nephelometry is not suitable at low concentrations

Concentration (µM)Std/Mean (%)

Based on standard regression fit, the calculated solubility limit for this compound is 51 µM, ten times more than the solubility limit measured with the EASY screener. The table tabulates the relative error (calculated from the N=5 replicates) for nephelometry. The relative error at low concentrations (<30 µM) is quite large (>5% and random) while the relative error at high concentration (>100 µM) is excellent at <5%. This table shows that nephelometry provides reproducible measurements at high concentrations but fails to produce reproducible measurements at low concentrations.

Reproducible and reliable measurements

To show the excellent reproducibility of the device, we measured the solubility limit of the same compound multiple times. For each experiment below, the compound is diluted from the same 10 mM DMSO stock solution and prepared on a weekly basis. The table shows the calculated solubility limit for each experiment. The solubility limit is taken as the concentration just before the normalized intensity is above 150% of the baseline (see horizontal black line).

Normalized Intensity

Concentration (µM)

Experiment #Solubility (µM)

With the EASY Screener

Solubility is no longer a speed breaker

Increased productivity

Getting quick and reliable solubility data allows you to plan your experiments efficiently

Improved decision making

You need not wait to make decisions based on reproducible solubility data. You can trust what you measure

Accelerated drug development pipeline

You remove the bottleneck in solubility measurement and prevent future solubility issues with proper testing



Oryl Photonics is an innovative Swiss company that develops laboratory devices based on proprietary light scattering technology. Oryl Photonics is a spin-off from Ecole Polytechnique Federal de Lausanne (EPFL) where the technology is incubated over a decade of research. The founders obtained their doctoral degree working on the technology

Orly Tarun

Orly Tarun, Ph.D

Founder, Sales & Marketing, Business Development, Prototyping
Nathan Dupertuis

Nathan Dupertuis, Ph.D

Founder, Sales & Marketing, Operations, Prototyping