EASY – Easy Assessment of SolubilitY

EASY Screener

Direct measure of aggregation for solubility
  • Simplified, cost-efficient & reliable solubility measurement
  • Maximize time savings
  • Avoid repetitive and labor-intensive work
  • Sustainable use of compounds & chemicals
  • Straightforward analysis

For faster, better and effective drug development!

Current prototype available for demonstration. Product to be released by Q1 2024.

As simple as nephelometry and as reliable as LC-MS

How does it work?

DILUTE
MEASURE
ANALYZE

Simplifying Solubility Measurement

State-of-the-art (LC-MS)

ORYL process

By removing the problematic steps, Easy saves

80%

TIME (5x)

95%

COST (20x)

99%

CHEMICALS (99x)

80%

COMPOUNDS (5x)

UN Sustainable Development Goals

H2O content (%)Std/Mean (%)
11.250.8%
33.750.7%
56.251.6%
78.752.5%
100 %2.3%

Direct measure of aggregation for solubility

What is the technology behind the EASY Screener?

The EASY screener is a light-scattering device that is different from the conventional linear scattering or the popular dynamic light scattering. The EASY screener is based on the physics of “nonlinear light scattering” where an ultrafast laser that generates very high peak intensities is used to excite the sample. In particular, the device is extremely sensitive to how substances (proteins, compounds, macromolecules) aggregate at the pre-aggregation level and is a direct measure of aggregation providing molecular level information. As proof of sensitivity we calibrate the device with mixtures of D2O (heavy water) and H2O (light water). The device is able to differentiate 21% change in D2O content. With this precision, detecting aggregation of small compounds is straightforward.

Sensitive to changes in molecular structure

Differentiates ~2% change of D2O

Designed to measure solubility

Easily handles small compounds (<1 nm) and beyond

Excellent repeatability & reproducibility

Relative error (std/mean) < 5% 

EASY is designed to measure the solubility limit

Comparison with nephelometry

We tested several compounds (in duplicate) that are poorly soluble (<50 uM solublity limit) and performed a direct comparison with nephelometry. As a metric, we use the relative error (std/mean) to determine the accuracy of the measurements.

The relative error for the EASY screener at low concentration is consistently low (std/mean) at <5% and increases when the compound is no longer soluble (aggregates are forming). In contrast, the opposite is true for nephelometry, the relative error is huge at low concentrations and start to decrease at high concentrations. This suggest that the EASY screener is highly sensitive in detecting the onset of aggregation (or pre-aggregation). This property allows EASY to detect the solubility limit with ease.

EASY SCREENER – Albendazole

Concentration (µM)Std/Mean (%)
0.12.84
12.77
2.53.05
53.86
1023.9
252.7 (saturated)

The EASY screener is able to detect solubility limit with ease. The jump in the normalized intensity (normalized to the average of the first two datapoints) is clearly visible. As reference, the response of a known compound that is soluble is shown. The table shows the relative error for each concentration.

NEPHELOMETRY – Albendazole

Concentration (µM)Std/Mean (%)
0.3-30
1429
3-48
10-22
3027
1001.9

The relative error of nephelometry at low concentrations (<30 uM) is huge while the the relative error at high concentration (>100) is acceptable at <5%. This suggests that nephelometry is sensitive to aggregates at high concentrations but provides unreliable measurements at low concentrations.

EASY SCREENER – Astemizole

Concentration (µM)Std/Mean (%)
0.12.57
12.02
2.51.68
52.38
103.30
255.50
5072.7

NEPHELOMETRY – Astemizole

Concentration (µM)Std/Mean (%)
0.3182.5
1183
3-45.8
10312
3068.3
10048.78
3002.46

EASY SCREENER – Curcumin

Concentration (µM)Std/Mean (%)
0.13.27
11.9
2.51.5
52.43
102.18
2511.07

NEPHELOMETRY – Curcumin

Concentration (µM)Std/Mean (%)
0.3343
1-171
3-72
10erroneous
30-595
10033
3005.1

EASY SCREENER – Fenofibrate

Concentration (µM)Std/Mean (%)
0.12.86
13
2.53.64
514.67
1017.26
2512.36

NEPHELOMETRY – Fenofibrate

Concentration (µM)Std/Mean (%)
0.3-20
1221
3100
10215
3084.2
10087.18
3001.53

Reproducible and reliable measurements

To show the excellent reproducibility of the device, we measured the solubility limit of the same compound multiple times. For each experiment below, the compound is diluted from the same 10 mM DMSO and prepared on a weekly basis. The table shows the calculated solubility limit for each experiment. When the normlized intensity is above 150% of the baseline, it signifies the formation of aggregates.

Experiment #Solubility (µM)
110
25
32.5
410
510
65
710
810
910

With the EASY SCREENER

Solubility is no longer a speed breaker

Increased Productivity

Getting quick and reliable solubility data allows you to plan your experiments efficiently

Improved decision making

You need not wait to make decisions based on reproducible solubility data. You can trust what you measure

Accelerates drug development pipeline

Remove the bottleneck in solubility measurement. Prevent future solubility issues with proper testing

OUR TEAM

WHO WE ARE

Oryl Photonics is an innovative Swiss company that develops laboratory devices based on proprietary light scattering technology. Oryl Photonics is a spin-off from Ecole Polytechnique Federal de Lausanne (EPFL) where the technology is incubated over a decade of research. The founders obtained their doctoral degree working on the technology

Orly Tarun

Orly Tarun, Ph.D

Founder, Sales & Marketing, Business Development, Prototyping
Nathan Dupertuis

Nathan Dupertuis, Ph.D

Founder, Sales & Marketing, Operations, Prototyping
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News & Stories

2022 in a nutshell

Oryl Photonics accomplished many things and reached new milestones in 2022: here is a wrap-up of the last year.

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