Current prototype available for demonstration. Product to be released by Q1 2024.
| H2O content (%) | Std/Mean (%) |
|---|---|
| 11.25 | 0.8% |
| 33.75 | 0.7% |
| 56.25 | 1.6% |
| 78.75 | 2.5% |
| 100 % | 2.3% |
The EASY screener is a light-scattering device that is different from the conventional linear scattering or the popular dynamic light scattering. The EASY screener is based on the physics of “nonlinear light scattering” where an ultrafast laser that generates very high peak intensities is used to excite the sample. In particular, the device is extremely sensitive to how substances (proteins, compounds, macromolecules) aggregate at the pre-aggregation level and is a direct measure of aggregation providing molecular level information. As proof of sensitivity we calibrate the device with mixtures of D2O (heavy water) and H2O (light water). The device is able to differentiate 21% change in D2O content. With this precision, detecting aggregation of small compounds is straightforward.
Differentiates ~2% change of D2O
Easily handles small compounds (<1 nm) and beyond
Relative error (std/mean) < 5%
We tested several compounds (in duplicate) that are poorly soluble (<50 uM solublity limit) and performed a direct comparison with nephelometry. As a metric, we use the relative error (std/mean) to determine the accuracy of the measurements.
The relative error for the EASY screener at low concentration is consistently low (std/mean) at <5% and increases when the compound is no longer soluble (aggregates are forming). In contrast, the opposite is true for nephelometry, the relative error is huge at low concentrations and start to decrease at high concentrations. This suggest that the EASY screener is highly sensitive in detecting the onset of aggregation (or pre-aggregation). This property allows EASY to detect the solubility limit with ease.
EASY SCREENER – Albendazole
| Concentration (µM) | Std/Mean (%) |
|---|---|
| 0.1 | 2.84 |
| 1 | 2.77 |
| 2.5 | 3.05 |
| 5 | 3.86 |
| 10 | 23.9 |
| 25 | 2.7 (saturated) |
The EASY screener is able to detect solubility limit with ease. The jump in the normalized intensity (normalized to the average of the first two datapoints) is clearly visible. As reference, the response of a known compound that is soluble is shown. The table shows the relative error for each concentration.
NEPHELOMETRY – Albendazole
| Concentration (µM) | Std/Mean (%) |
|---|---|
| 0.3 | -30 |
| 1 | 429 |
| 3 | -48 |
| 10 | -22 |
| 30 | 27 |
| 100 | 1.9 |
The relative error of nephelometry at low concentrations (<30 uM) is huge while the the relative error at high concentration (>100) is acceptable at <5%. This suggests that nephelometry is sensitive to aggregates at high concentrations but provides unreliable measurements at low concentrations.
EASY SCREENER – Astemizole
| Concentration (µM) | Std/Mean (%) |
|---|---|
| 0.1 | 2.57 |
| 1 | 2.02 |
| 2.5 | 1.68 |
| 5 | 2.38 |
| 10 | 3.30 |
| 25 | 5.50 |
| 50 | 72.7 |
NEPHELOMETRY – Astemizole
| Concentration (µM) | Std/Mean (%) |
|---|---|
| 0.3 | 182.5 |
| 1 | 183 |
| 3 | -45.8 |
| 10 | 312 |
| 30 | 68.3 |
| 100 | 48.78 |
| 300 | 2.46 |
EASY SCREENER – Curcumin
| Concentration (µM) | Std/Mean (%) |
|---|---|
| 0.1 | 3.27 |
| 1 | 1.9 |
| 2.5 | 1.5 |
| 5 | 2.43 |
| 10 | 2.18 |
| 25 | 11.07 |
NEPHELOMETRY – Curcumin
| Concentration (µM) | Std/Mean (%) |
|---|---|
| 0.3 | 343 |
| 1 | -171 |
| 3 | -72 |
| 10 | erroneous |
| 30 | -595 |
| 100 | 33 |
| 300 | 5.1 |
EASY SCREENER – Fenofibrate
| Concentration (µM) | Std/Mean (%) |
|---|---|
| 0.1 | 2.86 |
| 1 | 3 |
| 2.5 | 3.64 |
| 5 | 14.67 |
| 10 | 17.26 |
| 25 | 12.36 |
NEPHELOMETRY – Fenofibrate
| Concentration (µM) | Std/Mean (%) |
|---|---|
| 0.3 | -20 |
| 1 | 221 |
| 3 | 100 |
| 10 | 215 |
| 30 | 84.2 |
| 100 | 87.18 |
| 300 | 1.53 |
To show the excellent reproducibility of the device, we measured the solubility limit of the same compound multiple times. For each experiment below, the compound is diluted from the same 10 mM DMSO and prepared on a weekly basis. The table shows the calculated solubility limit for each experiment. When the normlized intensity is above 150% of the baseline, it signifies the formation of aggregates.
| Experiment # | Solubility (µM) |
|---|---|
| 1 | 10 |
| 2 | 5 |
| 3 | 2.5 |
| 4 | 10 |
| 5 | 10 |
| 6 | 5 |
| 7 | 10 |
| 8 | 10 |
| 9 | 10 |
Solubility is no longer a speed breaker
Getting quick and reliable solubility data allows you to plan your experiments efficiently
You need not wait to make decisions based on reproducible solubility data. You can trust what you measure
Remove the bottleneck in solubility measurement. Prevent future solubility issues with proper testing
Oryl Photonics is an innovative Swiss company that develops laboratory devices based on proprietary light scattering technology. Oryl Photonics is a spin-off from Ecole Polytechnique Federal de Lausanne (EPFL) where the technology is incubated over a decade of research. The founders obtained their doctoral degree working on the technology
Oryl Photonics accomplished many things and reached new milestones in 2022: here is a wrap-up of the last year.
Le 8 novembre dernier, notre co-fondateur Nathan Dupertuis s’est rendu à l’Université de Technologie d’Eindhoven pour présenter notre startup.
On November 8th, our co-founder Nathan Dupertuis went to Eindhoven University of Technology to pitch our startup.