EASY is designed to measure the solubility limit
Comparison with nephelometry
We tested several compounds (in duplicate) that are poorly soluble (<50 uM solublity limit) and performed a direct comparison with nephelometry. As a metric, we use the relative error (std/mean) to determine the accuracy of the measurements.
The relative error for the EASY screener at low concentration is consistently low (std/mean) at <5% and increases when the compound is no longer soluble (aggregates are forming). In contrast, the opposite is true for nephelometry, the relative error is huge at low concentrations and start to decrease at high concentrations. This suggest that the EASY screener is highly sensitive in detecting the onset of aggregation (or pre-aggregation). This property allows EASY to detect the solubility limit with ease.